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Preliminary Screening of Ayurvedic Formulation for Anticonvulsant Activity

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Journal name: Ancient Science of Life
Original article title: Preliminary Screening of a Classical Ayurvedic Formulation for Anticonvulsant Activity
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
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Original source:

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Author:

Arnab Dhar, Santosh Kumar Maurya, Ashish Mishra, Gireesh Kumar Singh, Manoj Kumar Singh, Ankit Seth


Ancient Science of Life:

(A quarterly multi-disciplinary scientific research journal in Ayurveda)

Full text available for: Preliminary Screening of a Classical Ayurvedic Formulation for Anticonvulsant Activity

Year: 2016

Copyright (license): CC BY-NC-SA


Summary of article contents:

Introduction

Epilepsy is a prevalent and complex neurological disorder characterized by recurrent convulsive seizures resulting from an imbalance between excitatory (glutamatergic) and inhibitory (GABAergic) neurotransmitters in the brain. Traditional pharmacological treatments often fail to control seizures effectively and can lead to undesired side effects, which increases the need for alternative therapies. Herbal medicines, particularly those detailed in traditional systems like Ayurveda, have been explored for their potential anticonvulsant properties. This study aimed to evaluate the anticonvulsant activity of a polyherbal formulation mentioned in Ayurvedic texts, specifically the "󲹾ṣaⲹ Ratnāvali," against seizures induced by both Maximal Electroshock and Pentylenetetrazole in rats.

The Role of Antioxidants in Anticonvulsant Activity

The study revealed that the polyherbal formulation (PHF), comprised of plants such as Terminalia chebula, Asparagus racemosus, and several others, exhibited protective effects against epilepsy. Notably, treatment with PHF significantly enhanced antioxidant enzyme activities, including superoxide dismutase (SOD) and glutathione (GSH) levels, while concurrently reducing lipid peroxidation (measured by malondialdehyde or MDA levels) in the brain. Furthermore, PHF decreased the activity of GABA-T, an enzyme responsible for GABA breakdown, thereby improving GABA levels in the brain. These findings underscore the importance of oxidative stress and its management through antioxidant mechanisms in developing effective anticonvulsant treatments.

Conclusion

This investigation highlights the potential efficacy of a traditional Ayurvedic polyherbal formulation in managing epilepsy by alleviating oxidative stress and modulating GABAergic neurotransmission. The results suggest that the formulation could serve as an alternative therapeutic option for epilepsy, particularly for patients unresponsive to conventional pharmacological treatments. Further studies are warranted to explore the specific biochemical mechanisms of action and to assess the formulation's clinical applicability in managing seizure disorders.

FAQ section (important questions/answers):

What was the aim of the study on the Ayurvedic formulation?

The study aimed to evaluate the anticonvulsant activity of a polyherbal formulation containing various herbs in rats, assessing its effectiveness against convulsions induced by Maximum electroshock and Pentylenetetrazole.

Which techniques were used to evaluate anticonvulsant activity?

The anticonvulsant activity was evaluated using Maximal Electroshock (MES) and Pentylenetetrazole (PTZ) induced convulsions in rats, following proper statistical methodologies for comparison.

What were the results related to oxidative stress markers?

The formulation significantly reduced lipid peroxidation levels and improved antioxidant enzyme activities (SOD and GSH) in the brain compared to control groups, indicating its potential to alleviate oxidative stress.

What conclusion was drawn regarding the polyherbal formulation's efficacy?

The findings suggest that the polyherbal formulation may be effective in treating epilepsy, potentially through GABAergic mechanisms, and shows promise as a safer alternative to conventional antiepileptic drugs.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Preliminary Screening of Ayurvedic Formulation for Anticonvulsant Activity�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
In the context of this research, activity refers to the biological effects induced by the polyherbal formulation on seizure control. It encompasses the evaluation of anticonvulsant properties, specifically demonstrating how effectively the formulation can mitigate or prevent seizures in animal models, which is crucial for its potential use in treating epilepsy.

2) Epilepsy:
Epilepsy is a chronic neurological disorder characterized by recurrent, unprovoked seizures due to abnormal electrical activity in the brain. It poses significant challenges to treatment and quality of life. The study presented aims to explore alternative treatment options, particularly those derived from traditional medicinal systems like Ayurveda, for managing epilepsy.

3) Drug:
In this research, a drug encompasses any chemical substance used for therapeutic purposes, particularly those administered to manage epilepsy. The study assesses the efficacy and safety of traditional herbal formulations as potential drug alternatives, highlighting the importance of exploring herbal medicines alongside conventional pharmacotherapy for treating seizures.

4) Channel:
In neuroscience, channels refer to protein structures that facilitate or inhibit the flow of ions across neural membranes, influencing neuronal excitability. These channels play a vital role in the action of antiepileptic drugs, as many pharmaceuticals target specific ion channels to prevent seizure spread in epilepsy management.

5) Ayurveda (Ayus-veda):
Ayurveda is a traditional Indian system of medicine that emphasizes a holistic approach to health, incorporating herbs, diet, and lifestyle changes. This study investigates Ayurvedic formulations as potential treatments for epilepsy, showcasing how historical practices can offer modern therapeutic alternatives and insights for managing neurological disorders.

6) Science (Scientific):
The term science refers to the systematic study of the natural world through observation and experimentation. In this research, scientific principles are applied to explore the pharmacological properties of Ayurvedic formulations, bridging traditional knowledge with modern scientific inquiry to validate their efficacy in treating convulsive disorders.

7) Water:
Water serves as a fundamental solvent in biological systems and is essential for various reactions within the body, including those related to drug absorption. In the context of herbal extraction processes, water is often utilized as a solvent for optimal extraction of active compounds from plants, enhancing the bioavailability of the therapeutic ingredients.

8) Mishra (Misra):
Mishra likely refers to Ashish Mishra, one of the authors contributing to this research. His involvement brings expertise in the field, covering essential aspects such as the design, formulation, or analysis of the herbal products being studied. His work helps to substantiate the claims made regarding the efficacy of the formulations.

9) Pharmacological:
Pharmacological pertains to the branch of medicine focused on drugs and their effects on biological systems. This research emphasizes pharmacological evaluations of the traditional herbal formulation, assessing its potential therapeutic effects and mechanisms behind its anticonvulsant properties, thereby contributing to the understanding of drug efficacy in epilepsy treatment.

10) Embelia ribes:
Embelia ribes is a medicinal plant widely recognized in Ayurveda for its anticonvulsant properties. The study investigates the efficacy of this herb within the polyherbal formulation, highlighting its role in potentially enhancing seizure control based on traditional uses and previous research that supports its therapeutic benefits.

11) Medicine:
Medicine encompasses various practices, including the use of drugs, surgery, and alternative therapies, aimed at maintaining or restoring health. This research contributes to the field of medicine by evaluating herbal formulations derived from traditional practices, aiming to provide safer and more effective treatment options for epilepsy.

12) Simha:
Sinha likely refers to S. Sinha, another author credited in the study. His contributions provide valuable insights into the research, possibly enhancing the credibility and depth of the study. The collaborative effort from multiple authors strengthens the research findings by incorporating diverse areas of expertise.

13) Terminalia chebula:
Terminalia chebula is another important medicinal plant included in the research. Known for its various health benefits, it is traditionally used in Ayurvedic medicine. This study evaluates its role and effectiveness within the polyherbal formulation, contributing to a comprehensive understanding of its potential in treating epilepsy.

14) Saussurea lappa:
Saussurea lappa, recognized for its medicinal properties, is included in the polyherbal formulation explored in this study. Its historical uses in traditional medicine provide a foundation for evaluating its anticonvulsant effects, thus contributing to the overall goal of identifying effective treatments for epilepsy.

15) Acorus calamus:
Acorus calamus, also known as sweet flag, is a known medicinal plant used in various traditional systems for its psychoactive and therapeutic properties. Its inclusion in the formulation under study aims to assess emergent effects on seizure prevention and management, focusing on its historical significance.

16) Tinospora cordifolia:
Tinospora cordifolia, an important component of traditional medicine, is known for its immune-boosting and neuroprotective properties. The research assesses its contributions to the overall effectiveness of the polyherbal formulation, providing insights into its potential role within the context of epilepsy treatment.

17) Asparagus racemosus:
Asparagus racemosus is a revered herb in traditional medicine, often used for its adaptogenic and restorative properties. In the context of this research, it is evaluated for its potential anticonvulsant effects, reinforcing the idea that herbal remedies can provide supportive roles in managing neurological disorders.

18) Depression:
Depression is a common comorbidity in patients with epilepsy, exacerbating their condition and reducing quality of life. This study indirectly addresses the issue by exploring herbal remedies that might alleviate both seizures and associated psychological burdens such as depression, thus highlighting the importance of holistic treatment approaches.

19) Bhaishajya (Bhaisajya):
󲹾ṣaⲹ refers to medicinal formulations or pharmaceutical aspects within the context of Ayurvedic literature. The study utilizes this concept to investigate traditional formulations for modern clinical applicability, thereby bridging cultural practices with scientific validation in the pursuit of effective remedies for epilepsy.

20) Quality:
Quality in this research pertains to the efficacy and safety of the polyherbal formulation being studied for treating epilepsy. Ensuring high quality in herbal medicines is vital for establishing their credibility and effectiveness. This study highlights the importance of maintaining quality control throughout the formulation and testing processes.

21) Anxiety:
Anxiety is commonly experienced by patients with epilepsy, who may fear seizures and their consequences. The study's exploration of herbal treatments may encompass anxiety management as a secondary benefit, indicating a comprehensive approach to treating not just seizures but the psychological impacts associated with epilepsy.

22) Vaca:
Vacha is another name for Acorus calamus, a plant traditionally used for its cognitive and neurological benefits. The study evaluates its inclusion in the formulation as a therapeutic agent for epilepsy, emphasizing its historical context and its relevance in contemporary medicinal practices.

23) Cina:
China, while not directly included in the study, may signify the importance of understanding varying traditional medicinal practices across cultures, including the therapeutic applications and efficacy of herbal compounds in managing diseases like epilepsy. This perspective provides a broader context for evaluating ancient remedies.

24) Glycyrrhiza glabra:
Glycyrrhiza glabra, also known as licorice, is recognized for its medicinal properties. Its possible role in the polyherbal formulation may contribute to its overall efficacy in treating epilepsy, highlighting the importance of diverse plant sources in developing effective therapeutic strategies.

25) Indigofera tinctoria:
Indigofera tinctoria is a plant traditionally used for its pharmacological properties. The study may explore its potential effects within the herbal formulation, contributing to a broader understanding of various plants that can collectively enhance the anticonvulsant activity of the formulation being examined.

26) Perception:
Perception from a neurological standpoint involves sensory processes that can be affected by seizures or related conditions. Understanding how treatments, including herbal formulations, influence perception can provide insights into improving the cognitive aspects of epilepsy treatment when considering overall patient welfare.

27) Discussion:
Discussion in the scientific context refers to the segment of research that critically analyzes findings, interpretations, and implications. This section critically evaluates results related to the polyherbal formulation's anticonvulsant effects, contributing to the understanding of its practical applications in treating seizure disorders.

28) Toxicity:
Toxicity pertains to the potential harmful effects of substances, including herbal formulations. This study likely evaluates the safety profile of the polyherbal formulation, aiming to ensure that the benefits of using the formulation in epilepsy treatment outweigh any potential risks associated with its consumption.

29) Siddha:
Siddha is another traditional system of medicine from India that focuses on holistic healing and comprises herbal remedies and practices. Its mention in related contexts emphasizes the importance of integrating knowledge from various traditional medical systems in the pursuit of effective treatments for neurological disorders like epilepsy.

30) Borax:
Borax, though primarily a cleaning agent, can indicate the importance of understanding various compounds in scientific research. Its relevance might arise in specific extraction processes or as a reference point for assessing the properties of active ingredients in herbal formulations, enhancing the study's analytical rigor.

31) Yoga:
Yoga represents a holistic practice that incorporates physical postures, breathing exercises, and mediation, often beneficial for individuals with epilepsy. Its inclusion may highlight complementary treatment strategies alongside herbal formulations, providing added benefits in managing seizures while promoting overall well-being.

32) Post:
Post signifies the conclusion or aftermath of the research process, denoting the summarization and dissemination of findings. In scientific studies, particularly those involving complex formulations, the 'post' might refer to the necessary steps for publishing results and encouraging further exploration and validation of traditional medicine.

Other Science Concepts:

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Discover the significance of concepts within the article: �Preliminary Screening of Ayurvedic Formulation for Anticonvulsant Activity�. Further sources in the context of Science might help you critically compare this page with similair documents:

Treatment of epilepsy, Herbal medicine, Polyherbal formulation, Traditional systems of medicine, Neurological disorder, Anticonvulsant activity, Superoxide dismutase, Ayurvedic classic, Institute of Medical Sciences, Classical Ayurvedic formulation, Ancient Science of life, Dose-dependent effect, Antioxidant enzyme, Excitatory neurotransmitter, Pentylenetetrazole-induced convulsion, Glutathione level, Epileptic patients, Oxidative stress marker.

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