Tyrosine kinase and PKC in J774A.1 macrophages by Tinospora cordifolia
Journal name: Journal of Ayurveda and Integrative Medicine
Original article title: Involvement of tyrosine-specific protein kinase and protein kinase C in J774A.1 macrophage functions activated by Tinospora cordifolia
The Journal of Ayurveda and Integrative Medicine (JAIM) is an open-access publication promoting collaboration between Ayurveda, traditional medicine, and biomedicine, publishing research on integrative health sciences
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Original source:
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Priti More, Kalpana Pai
Journal of Ayurveda and Integrative Medicine:
(A monthly peer-reviewed publication)
Full text available for: Involvement of tyrosine-specific protein kinase and protein kinase C in J774A.1 macrophage functions activated by Tinospora cordifolia
Year: 2017 | Doi: 10.1016/j.jaim.2016.12.007
Copyright (license): CC BY-NC-ND 4.0
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Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Tyrosine kinase and PKC in J774A.1 macrophages by Tinospora cordifolia�. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Study (Studying):
The term 'study' refers to the systematic investigation of a subject, which in this context implies the investigation of Tinospora cordifolia (guduchi) as a biological response modifier affecting macrophage activation and its implications for immunity and cancer treatment. Research is vital for drawing scientific conclusions.
2) Activity:
'Activity' in this context pertains to the biochemical and immunological actions of macrophages when stimulated by agents like guduchi. It denotes the enhanced functions of macrophages, such as increased production of nitric oxide (NO) and tumor necrosis factor (TNF-α), which are critical for immune response.
3) Medium:
The term 'medium' refers to the culture medium which provides nutrients and a suitable environment for cell growth during the experiments. Dulbecco's Modified Eagle Medium (DMEM) is specifically mentioned as the medium used to grow macrophage cell lines for assessing the effect of Guduchi.
4) Line:
'Line' usually signifies a lineage or type, particularly in biological contexts, such as cell lines derived from specific organisms. In this study, the J774A.1 macrophage cell line denotes a cultured source of macrophages used for evaluating the immune-modulatory effects of different substances.
5) Water:
In scientific research, 'water' is commonly used as a solvent and for preparing buffers. In this study, distilled water is referenced for preparing aqueous solutions necessary for cell culture and assays, underlining its crucial role in biological assays and maintaining sterile conditions.
6) Tinospora cordifolia:
'Tinospora cordifolia,' commonly known as guduchi, is the focus of this study as a natural immunomodulator. Its therapeutic properties in stimulating macrophage activity and enhancing immune responses bridge traditional medicine with modern biomedical applications, making it a subject of scientific investigation.
7) Antibiotic (Antibacterial):
The term 'antibiotic' relates to substances that inhibit bacterial growth or kill bacteria. In this context, the study compares natural biological response modifiers, like guduchi, with traditional antibiotics, aligning with the exploration of alternative therapeutic agents to enhance immunity rather than solely focusing on infection treatment.
8) Toxicity:
'Toxicity' refers to the degree to which a substance can harm organisms. The low toxicity of guduchi reinforces its potential as a safe therapeutic agent, particularly in cancer treatment and immune modulation, contrasting it with many synthetic drugs that may have harmful side effects.
9) India:
'India' is referenced as the country where guduchi is sourced and where the study is conducted. It underscores the geographical relevance of traditional herbs in Indian medicine, emphasizing the cultural contexts and rich biodiversity that informs the study of natural products.
10) Eagle:
'Eagle' refers to the modification of the original Eagles's medium, highlighting the importance of Eagle's formulations in cell biology. This modified medium supports optimal growth conditions for cultured cells, ensuring the reliability of experimental results that hinge on precise and controlled environments.
11) Pune:
'Pune' is a city in India where the study's cell lines were obtained, emphasizing the local biomedical infrastructure that supports the research. The city is noted for its academic and research institutions, making it a hub for scientific exploration and development.
12) Accumulation (Accumulating, Accumulate):
'Accumulating' implies the gradual gathering of evidence or knowledge. In this text, it suggests the ongoing research into the role of various compounds, particularly genistein and H-7, in macrophage activation, indicating a growing body of evidence supporting these pathways in immunology.
13) Maharashtra (Maharastra, Maha-rashtra):
'Maharashtra' is the Indian state from which Tinospora cordifolia is sourced, underlining the regional specifics of traditional medicine practices. It situates the study within a cultural context that values the use of indigenous plants for their health benefits.
14) Measurement:
'Measurement' pertains to the quantification of biological activity, such as determining levels of nitric oxide and TNF-α in experiments. This term highlights the methodological rigor applied in the study, emphasizing the importance of accurate data collection for validation of experimental hypotheses.
15) Developing:
'Developing' speaks to the ongoing process of understanding and refining knowledge in scientific research. It suggests that the mechanisms underlying macrophage activation and the effect of biological response modifiers like guduchi are still being explored, indicating a field ripe for further investigation.
16) Science (Scientific):
'Science' embodies the systematic methodology for investigation and understanding phenomena through observation and experimentation. This term solidifies the study's foundation in scientific principles, emphasizing the objective approach taken to elucidate the effects of guduchi on immune cells.
17) Company:
In this context, 'company' refers to the Sigma Chemical Company, a supplier of reagents and chemicals used in the experiments. Its mention underscores the reliance on commercial entities for high-quality, analytical-grade materials crucial for reproducible laboratory research.
18) Cancer:
'Cancer' is referenced to contextualize the relevance of macrophage activation in tumor biology. The investigation into guduchi's effects potentially aligns with immunotherapeutic strategies for cancer treatment, highlighting the significance of enhancing the immune response against malignant growths.
19) Nature:
'Nature' indicates the inherent properties of biological materials or organisms. In this study, it reflects on the natural origin of substances like guduchi and their roles in modulating immune responses, suggesting an integration of traditional knowledge and scientific inquiry into natural therapies.
Other Science Concepts:
Discover the significance of concepts within the article: �Tyrosine kinase and PKC in J774A.1 macrophages by Tinospora cordifolia�. Further sources in the context of Science might help you critically compare this page with similair documents:
Guduchi, MTT assay, Cytotoxic activity, Cell viability assay, Immunomodulating action, Endotoxin contamination, Macrophage activation, Biological response modifiers, Tumor necrosis factor, Culture supernatant, Nitric oxide production, Protein kinase C, First Line of Defense, Bi-directional interaction, Reactive nitrogen intermediates.