Significance of Serial dilution technique
The serial dilution technique is a laboratory method used to progressively dilute a sample in a controlled manner to determine the minimum inhibitory concentration (MIC) of a substance or microorganism. This technique helps establish a range of concentrations to assess the effectiveness against bacteria and is useful in isolating specific strains by reducing microbial concentrations. It facilitates counting colonies grown on agar plates and is essential for analyzing microbial populations in various research contexts.
Synonyms: Dilution series
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
The concept of Serial dilution technique in scientific sources
The Serial dilution technique is a laboratory method employed to isolate food-grade fungal cultures by diluting samples in sterile solutions. This method effectively reduces microbial concentration, facilitating easier counting, analysis, and isolation of specific strains.
From: World Journal of Pharmaceutical Research
(1) A method used to obtain 70 actinomycetes isolates from soil samples for further analysis in the study.[1] (2) A method used to prepare a series of solutions with decreasing concentrations, employed in antimicrobial testing.[2] (3) A method used to reduce the concentration of a substance in a solution, utilized here for isolating microorganisms.[3] (4) A method of serially reducing the concentration of a sample to estimate microbial populations.[4] (5) A method used to create a range of concentrations of a compound to determine the minimum inhibitory concentration against bacteria.[5]
From: Ancient Science of Life
(1) A method used to determine the MIC by progressively diluting the crude extract in a controlled manner.[6] (2) A method used to determine the concentration of a substance or microorganism by diluting a sample in a stepwise manner, performed in the study to establish MIC.[7]