Significance of Agar well diffusion method
The Agar well diffusion method is a microbiological technique used to evaluate the antibacterial activity of various substances by measuring zones of inhibition on agar plates. It involves creating wells in the agar medium inoculated with bacteria and observing the effect of test substances on microbial growth. This method is widely utilized to assess the effectiveness of antimicrobial agents, including extracts from plants, in preventing bacterial and fungal growth, thereby providing valuable insights into their potential applications in combating infections.
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The concept of Agar well diffusion method in scientific sources
The Agar well diffusion method is a laboratory technique for assessing antimicrobial susceptibility and antibacterial activity by measuring zones of inhibition on agar plates surrounding wells containing test substances.
From: The Malaysian Journal of Medical Sciences
(1) The agar well diffusion method is a technique used to assess the antibacterial properties of substances, where the substance diffuses into the agar medium and inhibits bacterial growth.[1]